- Global analysis of immune evasion strategies in Staphylococcus aureus clinical isolates
- Brignoli, Tarcisio <1989>
Subject
- BIO/11 Biologia molecolare
Description
- Staphylococcus aureus is a major human pathogen, leading cause of soft tissue and blood stream infections. One of the causes of its success as a pathogen is the peculiar array of immune evasion factors that enables the bacterium to avoid host defenses, in which the staphylococcal protein A (SpA) plays a major role thanks to its IgG binding activities. Moreover, SpA has been recently proposed as a promising vaccine antigen. In this study, we evaluated the expression of SpA in a collection of staphylococcal strains, among which about 7% did not express SpA (SpA- strains), despite the presence of the gene. By a comparative genomic analysis, we identified that a mutation in the spa 5’ UTR sequence affecting the RBS is responsible for the loss of SpA in a subset of SpA- strains. Using a high-throughput qRT-PCR approach on a selected panel of virulence-related genes, we identified that the SpA- phenotype is associated with lower spa transcript levels and increased expression and production of capsule as well as other changes in the transcription of several key virulence factors. Our data suggest that the SpA- phenotype has occurred in geographically distinct strains through different molecular mechanisms including both transcription and translation alterations. Furthermore, we provide evidence that the SpA- strains are highly susceptible to phagocytic uptake mediated by anti-capsule antibodies. These data suggest that S. aureus may alter its virulence factor expression pattern as an adaptation to the host or environment. Vaccination strategies targeting both SpA and capsule could therefore result in broader coverage against staphylococcal isolates.
Date
- 2019-04-03
Type
- Doctoral Thesis
- PeerReviewed
Format
- application/pdf
Identifier
urn:nbn:it:unibo-24905
Brignoli, Tarcisio (2019) Global analysis of immune evasion strategies in Staphylococcus aureus clinical isolates, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Biologia cellulare e molecolare