- Cellular and molecular effects of cross reacting material 197 (CRM197) on HT-29 colon cancer cell line
- Rivetti, Stefano <1983>
Subject
- BIO/17 Istologia
Description
- Cross Reacting Material 197(CRM197) is a Diphteria toxin non toxic mutant that had shown anti-tumor activity in mice and humans. CRM197 is utilized as a specific inhibitor of heparin-binding epidermal growth factor (HB-EGF), that competes for the epidermal growth factor receptor (EGFR), overexpressed in colorectal cancer and implicated in its progression. We evaluated the effects of CRM197 on HT-29 human colon cancer cell line behaviour and, for CRM197 recognized ability to inhibit HB-EGF, its possible effects on EGFR activation. In particular, while HT-29 does not show any reduction of viability after CRM197 treatment, or changes in cell cycle distribution, in EGFR localization or activation, they show a change in gene expression profile analyzed by microarray. This is the first study where the CRM197 treatment on HT-29 show the alteration of a specific and selected number of genes.
Date
- 2012-05-15
Type
- Doctoral Thesis
- PeerReviewed
Format
- application/pdf
Identifier
urn:nbn:it:unibo-4315
Rivetti, Stefano (2012) Cellular and molecular effects of cross reacting material 197 (CRM197) on HT-29 colon cancer cell line, [Dissertation thesis], Alma Mater Studiorum Università di Bologna. Dottorato di ricerca in Scienze dello sviluppo e del movimento umano: progetto n. 2 "Sviluppo e riproduzione umana"